Current and emerging assays for Francisella tularensis detection: a review
نویسندگان
چکیده
This paper presents an overview of methods for detection and identification of the pathogenic bacterium Francisella tularensis such as cultivation tests, enzyme-linked immunosorbent assays, flow cytometry, polymerase chain reaction, immunosensor, microarray, mass spectrometry, and chromatography. Included references are chosen according to their practical importance or perspectives for the future.
منابع مشابه
Diversity and Evolution of Francisella tularensis
Francisella tularensis has been the focus of much research over the last two decades mainly because of its potential use as an agent of bioterrorism. F. tularensis is the causative agent of zoonotic tularemia and has a worldwide distribution. The different subspecies of F. tularensis vary in their biogeography and virulence, making early detection and diagnosis important in both the biodefense ...
متن کاملSimple and Rapid Detection of Yersinia Pestis and Francisella Tularensis using Multiplex-PCR
Background: Yersinia pestis and Francisella tularensis cause plague and tularemia, which are known as diseases of the newborn and elderly, respectively. Immunological and culture-based detection methods of these bacteria are time-consuming, costly, complicated and require advanced equipment. We aimed to design and synthesize a gene structure as positive control for molecular detection of these ...
متن کاملDetection of diverse new Francisella-like bacteria in environmental samples.
Following detection of putative Francisella species in aerosol samples from Houston, Texas, we surveyed soil and water samples from the area for the agent of tularemia, Francisella tularensis, and related species. The initial survey used 16S rRNA gene primers to detect Francisella species and related organisms by PCR amplification of DNA extracts from environmental samples. This analysis indica...
متن کاملRapid Identification and Characterization of Francisella by Molecular Biology and Other Techniques
Francisella tularensis is the causative pathogen of tularemia and a Tier 1 bioterror agent on the CDC list. Considering the fact that some subpopulation of the F. tularensis strains is more virulent, more significantly associated with mortality, and therefore poses more threat to humans, rapid identification and characterization of this subpopulation strains is of invaluable importance. This re...
متن کاملCoxiella burnetii in Ticks, Argentina
6. Janse I, Hamidjaja RA, Bok JM, van Rotterdam BJ. Reliable detection of Bacillus anthracis, Francisella tularensis and Yersinia pestis by using multiplex qPCR including internal controls for nucleic acid extraction and amplification. BMC Microbiol. 2010;10:314. 7. Adjemian JZ, Adjemian MK, Foley P, Chomel BB, Kasten RW, Foley JE. Evidence of multiple zoonotic agents in a wild rodent community...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2008